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S-BIO confidently offers an antibody (IgG) N-glycan sample preparation kit compatible with capillary electrophoresis (CE). This kit allows to start from purified IgG or unpurified IgG (such as the culture supernatant of CHO cells), and perform IgG immobilization, N-glycan release, fluorescence labeling and clean-up all in one tube, enabling quick and simple preparation of glycan samples for CE analysis. The kit includes a glycan release enzyme and a labeling reagent (APTS). Moreover, the labeling reaction does not use hazardous cyanide compounds, making it a user-friendly kit. |
P/ACE MDQ Plus (AB Sciex)
Capillary: N-CHO capillary (AB Sciex, 477601)
Buffer: N-Linked Carbohydrate Separation Gel Buffer (AB Sciex, 477623)
Sequence: Shown in the table
Fluorescent detection: Ex. 488 nm, Em. 520 nm
Table: Analysis sequence
A mixture of human serum IgG (40 μg) in the culture supernatant (120 μL) of HEK293 cells was treated with the EZGlyco® mAb-N Kit with APTS to prepare APTS-labeled N-glycan samples. Purified human serum IgG was treated in the same way as a control. CE analysis was performed under the conditions described.
The same peak pattern was obtained with the IgG spiked in the culture supernatant and the purified IgG, demonstrating that this kit can be applied to both purified and unpurified antibodies.
(a) Trastuzumab deruxtecan and (b) Trastuzumab emtansine (40 μg) were treated with the EZGlyco® mAb-N Kit with APTS to prepare APTS-labeled N-glycan samples. CE analysis was performed under the conditions described.
Data demonstrated that this kit can be applied to not only naked IgG but also ADC N-glycan analysis.
Product No. | Product Name | Contents | Quantity | Price |
---|---|---|---|---|
BS-43607Z | Auto-EZGlyco® mAb-N Kit with APTS | Antibody capturing column Antibody capturing buffer Washing buffer PNGase F PNGase F enhancer APTS (8-aminopyrene-1,3,6-trisulfonic acid) Reducing reagent (non-toxic) Cleanup column Protocol booklet |
10 tests | ASK |
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