Exosome recovery comparison experiment
Exosomes, which are extracellular membrane vesicles, play an important role in intercellular signal transduction. They are also considered to be involved in cancer progression and metastasis. However, it is known that exosomes adhere to the wall surface of plastic tubes and affect the yield. It is important to efficiently recover exosomes when conducting exosome research. We investigated the recovery rate of exosomes using ProteoSaveTM and compared it with other products.
Material
Purified exosomes derived from PC-3 cells (System Biosciences)
CD63-Capture Human Exosome ELISA Kit (Fujifilm Wako)
ProteoSaveTM (MS-4265M), microtubes from different companies
Method
Exosomes were dispensed into tubes from each company, allowed to stand for 5 minutes, and then transferred to another tube 4 times. Changes in the amount of exosomes before and after the transfer process were examined. The amount of exosomes was measured.
Results
ć»Compared to other products, ProteoSaveTM showed less exosome adsorption
Compared to other products, ProteoSaveTM showed less exosome adsorption