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[Experiments] STEMFULL™
Cell recovery improvement of adhesive cells

Comparison data of Corneal epithelial cells

Data provided by Dr. Masakazu Yokoo, Dept. Ophthalmology, The University of Tokyo Hospital

• Corneal epithelial cells recovery

Corneal epithelial cells recovery from corneal endothelial tissue using STEMFULL compared to conventional tube.

Corneal epithelial cells recovery

• Colony-formation assay

Colony number comparison from recovered corneal epithelial cells.

Colony-formation assay

Comparison data of iPS-derived CD104 positive epithelial cells

Data provided by Dr. Ryuhei Hayashi, Stem Cells and Applied Medicine Lab, Osaka University Graduate School of Medicine

• Cell recovery experiment

20000 cells were suspended and cell recovery was evaluated after 2-hour storage at r.t.

STEMFULL gave higher cell recovery, especially in serum-free media.
STEMFULL, together with serum medium, remarkably reduced cell loss.

Cell recovery of iPS-derived CD104+ epithelial cells

• Colony-formation assay

2000 recovered cells were seeded on feeder cells (12-well plate). After 9 days of cultivation, cells were stained by 2% Rhodamine B solution and colony-forming efficiency (CFE) was determined.

Serun Free medium/Serum medium
Colony-formation assay

STEMFULL efficiently recovered stem cells,
and the effect was especially pronounced in serum-free medium.