Data provided by: Research and Development Center, Otsuka Pharmaceutical Factory, Inc.
Experiment 1
| Cell | ： | Human adipose-derived MSCs (hAD-MSCs) |
|---|---|---|
| Cell density | ： | 5×105 cells/mL |
| Method | ： | hAD-MSCs suspended in Cellstor-W or Cellstor-S were added into conventional or STEMFULL tube and stored at 5°C. Cell recovery and cell viability were evaluated over time. |
Solution: Cellstor-W
Mean ± SD (n=6), *; p<0.05, **; p<0.01 Student’s t test
No significance difference was found between 0 hours and other time points after storage in both tubes（Dunnett’s test）
Solution: Cellstor-S
Mean ± SD (n=4), *; p<0.05, **; p<0.01 Student’s t test
No significance difference was found between 0 hours and other time points after storage in both tubes（Dunnett’s test）
Storage of hAD-MSCs suspended in Cellstor-W or Cellstor-S at 5°C in STEMFULL maintained a high cell viability and a higher cell recovery rate for
at least 24 hours than storage in a conventional tube.
Experiment 2
| Cell | ： | Human adipose-derived MSCs (hAD-MSCs) |
|---|---|---|
| Cell density | ： | 5×105 cells/mL |
| Method | ： | hAD-MSCs suspended in Cellstor-W or Cellstor-S were added into conventional or STEMFULL tube and stored at 25°C. Cell recovery and cell viability were evaluated over time. |
Solution: Cellstor-W
Mean ± SD (0, 3, 6 h; n=4, 24 h; n=3), *; p<0.05, ***; p<0.001 Student’s t test
†; p<0.05, ††; p<0.01, 0 vs. other storage time (Dunnett’s test)
Solution: Cellstor-S
Mean ± SD (n=4), *; p<0.01, **; ***; p<0.001 Student’s t test
†; p<0.05, ††; p<0.01, †††; p<0.001, 0 vs. other storage time (Dunnett’s test)
Storage of hAD-MSCs suspended in Cellstor-W or Cellstor-S at 25°C in STEMFULL maintained a high cell viability.
Storage in Cellstor-W maintained a higher cell recovery rate for at least 24 hours
and in Cellstor-S for 6 hours than storage in a conventional tube.
Cellstor-S is a cell suspension and preservation solution, and Cellstor-W is a cell wash and preservation solution manufactured by Otsuka Pharmaceutical Factory, Inc.
※ They are reagents for research use and are not intended for medical use in humans or animals.
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