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  5. Oligosaccharide Purification Kit BlotGlycoTM

Oligosaccharide Purification Products Oligosaccharide Purification Kit BlotGlycoTM

Products

BlotGlycoTM beads are chemically synthesized polymer particles. High density hydrazide groups on BlotGlycoTM can bind specifically with aldehyde groups at the reducing end of glycans. As a result, BlotGlycoTM beads capture only glycans from the variety of biological samples selectively and comprehensively. The hydrazide groups forms covalent binding with aldehyde groups stably. Therefore, it enables to carry out the thorough washing steps to eliminate peptides and other impurities easily.

*Purification and labeling are completed in about 5 hours using just one spin tube.

*No special equipment is necessary except a heating block and a small desktop centrifuge. No column chromatography is necessary.

*Glycans can be purified and labeled from various crude biological samples. Conventional fluorescent labeling reagents (2AB, PA) are applicable. Kit contains a unique labeling reagent for the high sensitive MALDI-TOF MS measurement.

*By using a 96 well filter plate and a vacuum manifold, as many as 96 samples are processed simultaneously.

Medical Institution/Laboratory

Oligosaccharide Analysis

Oligosaccharide purification

Glycosylation analysis of biologics (for research, quality control and production process monitoring)

Glycosylation analysis of cells, especially for the differentiation researches of stem cells

Combination of glycomics and protemics (glycans can be analyzed from electrohoresis bands)

Cat. No Product Composition
BlotGlycoTM
Beads
Labeling reagent
for MALDI-TOF MS
Spin tube Clean up
column
BS-45603Z BlotGlycoTM10

for 10 time

assays
(50mg)

for 10 time assays 10 10
BS-45604Z BlotGlycoTM50

for 50 time

assays
(250mg)

for 50 time assays 50 50
BS-45605Z BlotGlycoTM100

for 100 time

assays
(500mg)

for 100 time assays 100 100
BS-45606Z BlotGlycoTM100A

for 100 time

assays
(500mg)

for 100 time assays - -
BS-45607Z BlotGlycoTM100B

for 100 time

assays
(500mg)

- 100 100
BS-45608Z BlotGlycoTM100C

for 100 time

assays
(50mg)

- - -
BS-45609Z BlotGlycoTM100D

for 100 time

assays
(500mg)

- - 100
BS-45611Z BlotGlycoTM100E

for 100 time

assays
(500mg)

- 100 -
BS-45610Z BlotGlycoTM200C

for 200 time

assays
(1000mg)

- - -

Remark

  • Storage: 4°C
  • aoWR is a special labeling reagent for MALDI-TOF MS.

Glycosylation analysis of Biologics

N-glycan of antibody: using 10μg IgG

Biomarker discovery

N-glycan of human serum: using 5μL serum

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S-BIO Business Division

Tel : +81-3-5462-4831
Fax : +81-3-5462-4835

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