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Low Protein Adsorption Products and ELISA Related Products
PROTEOSAVE™

Products

PROTEOSAVE™ is a product that blocks nonspecific adsorption of protein, peptide to the inside of a plastic container by the coating of ultra hydrophilic polymer that S-BIO has newly and originally developed.

Features

Reduce the adsorption of proteins, peptides and cells

The unique surface hydroxide group is coating and decreasing the loss of protein sample by preventing non-specific adsorption of protein and peptide.

Improved organic solvents resistance and heat resistance

PROTEOSAVE™ is stable against general used organic solvents, detergents and heating below 100°C.

Applications

Medical Research Institution/Laboratory

Protein Analysis

Adjustment/Preservation

  • To preserve cell expressed proteins and preparation of culture additives and proteins
  • To reduce loss of protein in a valuable sample

Specification

PROTEOSAVE™ (organic solvent resistant and heat resistant)
Cat. NoProductMaterialNoteQty/PkQty/Cs
MS-4205MZPROTEOSAVE™ 0.5mL MicrotubePolypropyleneNon-sterilized100500
MS-4255MZPROTEOSAVE™ 0.5mL Microtube
(Sterilized)
PolypropyleneRadiation sterilized100500
MS-4215MZPROTEOSAVE™ 1.5mL MicrotubePolypropyleneNon-sterilized100500
MS-4265MZPROTEOSAVE™ 1.5mL Microtube
(Sterized)
PolypropyleneRadiation sterilized100500
MS-4220MZPROTEOSAVE™ 2.0mL MicrotubePolypropyleneNon-sterilized100500
MS-4270MZPROTEOSAVE™ 2.0mL Microtube
(Sterized)
PolypropyleneRadiation sterilized100500
MS-4201XZPROTEOSAVE™ 0.5mL SlimtubePolypropyleneNon-sterilized50500
MS-4202XZPROTEOSAVE™ 2.0mL SlimtubePolypropyleneNon-sterilized50500

Remark

  • Storage: Room temperature
  • Expiration: 2 years after production
PROTEOSAVE™
Cat. NoProductMaterialNoteQty/PkQty/Cs
MS-52150ZPROTEOSAVE™
15mL Conicaltube*
Body: PET
Cap: Polyethylene
Non-sterilized5100
MS-52550ZPROTEOSAVE™
50mL Conicaltube
(Sterilized)
Body: Polypropylene
Cap: Polyethylene
Radiation sterilized5100
MS-8296FZPROTEOSAVE™
96-wells, Flat Plate
PolystyreneNo Lid,
Non-sterilized
550
MS-8296VZPROTEOSAVE™
96-wells, V Plate
PolypropyleneNo Lid,
Non-sterilized
520
MS-82962ZPROTEOSAVE™
96-deep wells, V Plate, 2mL
PolypropyleneNo Lid,
Non-sterilized
345
MS-8296KZPROTEOSAVE™
96-wells, Black, Flat Plate
PolystyreneNo Lid,
Non-sterilized
550
MS-3296UZPROTEOSAVE™
96-wells, U Plate
PolystyreneNo Lid,
Non-sterilized
550

Remark

  • Storage: Room temperature
  • Expiration: 2 years after production
  • *: Operational temperature -80ºC to 40ºC

Experiments

Application example in Proteomics Research

Comparison of the number of identified peptides with competitive products

Experimental conditions

Vessl ProteoSave™SS 1.5mL
Microtube
5 competitive
Sample Peptides derived from Hep38
Quantity 300 ng
Method nanoLC-Ultra 2D with TripleTOF® 5600, AB SCIEX
Column 75µm×150mm, ChromXP C18-CL, 3µm 120 Å, Ekisigent
Eluent A: 0.1% FA and 1% ACN
B: 0.1% FA and 99% ACN
Software Mascot Server
  • Provided by Dr. Masahiro Kamita, Ph.D, National Cancer Center Research Institute, JAPAN.

Application example in analysis of small molecules

Comparison of Non-ionic small compounds with non-coated tube

Experimental conditions

Vessel PriteoSave™SS 1.5mL Microtube
Non-coated 1.5mL Microtube
Concentration 0.1 µg/mL
Sample Non-ionic small molecule
1. Digoxin (Anti-anginal drug)
2. Paclitaxel (Anti-canter drug)
Meadurement After 1h incubation with non-ionic small molecule, determined the concentration with LC/MS/MS

Adsorption (%) = 100 - Concentration in the 0 and 1h sample / Concentration in the initial sample x 100

  • Provided by SEKISUI MEDICAL CO., LTD., JAPAN

Reference

Proteosave Microtube (Cat. No. MS-4205M,MS-4255M,MS-4215M,MS-4265M)

  • HAMAMURA, Kensuke, et al. ANNALS EXPRESS: Simple quantitation for potential serum disease biomarker peptides, primarily identified by a peptidomics approach in the serum with hypertensive disorders of pregnancy. Annals of Clinical Biochemistry: An international journal of biochemistry and laboratory medicine, 2015, 0004563215583697.
  • IZAKI, Shunsuke, et al. Feasibility of Antibody–Poly (Glutamic Acid) Complexes: Preparation of High-Concentration Antibody Formulations and Their Pharmaceutical Properties. Journal of pharmaceutical sciences, 2015, 104.6: 1929-1937.
  • ICHIKAWA, Shunsuke, et al. Cellulosomal carbohydrate-binding module from Clostridium josui binds to crystalline and non-crystalline cellulose, and soluble polysaccharides. FEBS letters, 2014.
  • KADOKAWA, Hiroya, et al. Gonadotropin-releasing hormone (GnRH) receptors of cattle aggregate on the surface of gonadotrophs and are increased by elevated GnRH concentrations. Animal reproduction science, 2014, 150.3: 84-95.
  • UCHIDA, Yasuo, et al. A study protocol for quantitative targeted absolute proteomics (QTAP) by LC-MS/MS: application for inter-strain differences in protein expression levels of transporters, receptors, claudin-5, and marker proteins at the blood–brain barrier in ddY, FVB, and C57BL/6J mice. Fluids and Barriers of the CNS, 2013, 10.1: 21.
  • NAGAI, Yutaka; TAKAO, Masashi. Monoclonal antibody to human epithelial cell adhesion molecule and method for detecting circulating tumor cells using the same. U.S. Patent Application 14/085,205, 2013.
  • TAKAO, Masashi; NAGAI, Yutaka; TORII, Tokiji. Cysteine-Poor Region-Specific EpCAM Monoclonal Antibody Recognizing Native Tumor Cells with High Sensitivity. Monoclonal antibodies in immunodiagnosis and immunotherapy, 2013, 32.2: 73-80.
  • YASUNO, K., et al. Development of Podocyte Injuries in Osborne–Mendel Rats is Accompanied by Reduced Expression of Podocyte Proteins. Journal of comparative pathology, 2013, 149.2: 280-290.
  • TSUCHIYA, Hikaru; TANAKA, Keiji; SAEKI, Yasushi. The parallel reaction monitoring method contributes to a highly sensitive polyubiquitin chain quantification. Biochemical and biophysical research communications, 2013, 436.2: 223-229.
  • KUROKAWA, Kenji, et al. Novel bacterial lipoprotein structures conserved in low-GC content Gram-positive bacteria are recognized by Toll-like receptor 2. Journal of Biological Chemistry, 2012, 287.16: 13170-13181.
  • UMEMURA, Hiroshi, et al. Identification of a high molecular weight kininogen fragment as a marker for early gastric cancer by serum proteome analysis. Journal of gastroenterology, 2011, 46.5: 577-585.
  • KAWAKAMI, Hirotaka, et al. Dynamics of absolute amount of nephrin in a single podocyte in puromycin aminonucleoside nephrosis rats calculated by quantitative glomerular proteomics approach with selected reaction monitoring mode. Nephrology Dialysis Transplantation, 2011, gfr492.
  • TAKAO, Masashi; TAKEDA, Kazuo. Enumeration, characterization, and collection of intact circulating tumor cells by cross contamination‐free flow cytometry. Cytometry Part A, 2011, 79.2: 107-117.
  • FUKUMOTO, Hiroaki, et al. High-molecular-weight β-amyloid oligomers are elevated in cerebrospinal fluid of Alzheimer patients. The FASEB Journal, 2010, 24.8: 2716-2726.
  • ICHIKAWA, S.; KARITA, S. Characterization of Family 3 Carbohydrate-binding Module from Clostridium josui. In: Proceedings of the Second International Workshop on Regional Innovation Studies: (IWRIS2010). Graduate School of Regional Innovation Studies, Mie University, 2010. p. 5-8.

Conical Tube (Cat. No. MS-52150,MS-52550)

  • WATANABE, H., et al. Controlled release of a protein using a ceramic carrier and zinc ions as a novel approach to the treatment of osteoporosis. In: Key Engineering Materials. 2015. p. 332-337.
  • IHARA, Yuta; OHTA, Hiroyuki; MASUDA, Shinji. A highly sensitive quantification method for the accumulation of alarmone ppGpp in Arabidopsis thaliana using UPLC-ESI-qMS/MS. Journal of plant research, 2015, 128.3: 511-518.
  • KIM, Jong-Myong, et al. Highly Reproducible ChIP-on-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana. In: Arabidopsis Protocols. Humana Press, 2014. p. 405-426.

Proteosave 96F Plate (Cat. No. MS-8296F)

  • FUKAZAWA, Tominaga; YAMAZAKI, Yuri; MIYAMOTO, Yohei. Reduction of non-specific adsorption of drugs to plastic containers used in bioassays or analyses. Journal of pharmacological and toxicological methods, 2010, 61.3: 329-333.

Proteosave 96F Plate (Black) MS-8296K

  • ANDOU, Takashi, et al. RNA detection using peptide-inserted Renilla luciferase. Analytical and bioanalytical chemistry, 2009, 393.2: 661-668.

Others

  • KASUGA, Kie. Comprehensive analysis of MHC ligands in clinical material by immunoaffinity-mass spectrometry. In: The Low Molecular Weight Proteome. Springer New York, 2013. p. 203-218.
  • YAMASHITA, Kazuyuki; SHIROKI, Masahiro. Medical or biochemical resin composition and resin molded product. U.S. Patent Application 13/469,768, 2012.
  • GOTOH, Akiko, et al. Evaluation of adsorption of urine cystatin C to the polymer materials on the microplate by an antigen capture enzyme-linked immunosorbent assay. Clinica Chimica Acta, 2008, 397.1: 13-17.

Exhibitions More

  • 2016/10/13 8th Asian Community of Glycoscience and Glycotechnology
  • 2016/05/17 7th Annual Biosimilars Asia 2016 Conference & Exhibition

Call or email us about our Division/Department.

TEL:
+81-3-5462-4831
Fax:
+81-3-5462-4835

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