Glycan Analysis Related Products
BlotGlyco™ Glycan Purification and Labeling Kit

Products

BlotGlyco™ is a sample preparation kit for glycan analysis of biological samples. Using this kit, it is possible to purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on. BlotGlyco™ beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it is possible to carry out the vigorous washing steps to eliminate peptide and other impurities. BlotGlyco™ kit is a quite useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.

We provide high throughput glycan analysis services based on BlotGlyco™ glycan purification.
For details, please visit the following website: http://www.s-bio.com/services/

Features

  • Purification and labeling are completed in about 5 hours using just one spin tube.
  • No special equipment is necessary except a heating block and a small desktop centrifuge. No column chromatography is necessary.
  • Glycans can be purified and labeled from various crude biological samples. Conventional fluorescent labeling reagents (2AB, PA) are applicable. Kit contains a unique labeling reagent for the high sensitive MALDI-TOF MS measurement.
  • By using a 96 well filter plate and a vacuum manifold, as many as 96 samples are processed simultaneously.

How to use BlotGlyco™ 96 Well Plate

Applications

Medical Research Institution/Laboratory

Sample Preparation for Glycan Analysis

Purpose of use

Glycosylation analysis of biologics (for research, screening, quality control and production process monitoring)
Glycosylation analysis of cells, especially for the differentiation researches of stem cells
Combination of glycomics and proteomics (glycans can be analyzed from electrohoresis bands)
Glycan biomarker discovery

Specifications

Cat. No. Product Contents
Polymer beads aoWR Reaction tube Cleanup column
BS-45403Z BlotGlyco™10 for 10 assays for 10 assays 10 10
BS-45404Z BlotGlyco™50 for 50 assays for 50 assays 50 50
BS-45405Z BlotGlyco™100 for 100 assays for 100 assays 100 100
BS-45406Z BlotGlyco™100A for 100 assays for 100 assays - -
BS-45407Z BlotGlyco™100B for 100 assays - 100 100
BS-45408Z BlotGlyco™100C for 100 assays - - -
BS-45409Z BlotGlyco™100D for 100 assays - - 100
BS-45410Z BlotGlyco™200C for 200 assays - - -
BS-45411Z BlotGlyco™100E for 100 assays - 100 -
BS-45412Z BlotGlyco™200E for 200 assays - 200 -
BS-45413Z BlotGlyco™96 plate B for 96 assays - 1 Filterplate 1 Cleanup plate
BS-45414Z BlotGlyco™10B for 10 assays - 10 10
BS-45415Z BlotGlyco™50B for 50 assays - 50 50

Remark

  • Storage: 4°C
  • aoWR is a special labeling reagent for MALDI-TOF MS.

Experiments

Glycosylation analysis of Biologics

N-glycan of antibody: using 10μg IgG

Biomarker discovery

N-glycan of human serum: using 5μL serum

Call or email us about our Division/Department.

TEL:
+81-3-5462-4831
FAX:
+81-3-5462-4835

※9:00-17:40 Mon.-Fri. (JST)

Please take a look at the FAQ first.