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Cell Culture Products Low Cell Adhesion Products "PrimeSurfaceTM"
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PrimeSurfaceTM prohibits cells from adhering to matrices based upon SUMILON's proprietary low-protein-adsorption technology. That makes an easy formation of cell spheroid colony |
Ideal for homogeneous cell spheroid formation
Adhesion of cell to culture surface is controlled so that spheroid formation ratio is high and homogeneous culture is possible.
Just fit for study on differentiation
Chemicals for differentiation induction can be added after EB body formation of ES cells.
No elution of chemicals from substrate
Stable culture surface due to chemical bonding of ultra-hydrophilic coating polymer makes no elution of chemicals from substrate.
Ideal for fifferenttiation and stimulation responce studies
Ideal for differentiation and stimulation response studies due to the near in vivo environment by eliminating influence of the plate material on cells.
Medical institution/Laboratory
Cell Culture
Can be used for induction of ES cells/IPS cells and nerve stem cells differentiation and drug design screening by three dimensions model of cells
Culture example using PrimeSurfaceTM96U
Mouse ES cell culture
| Seeding cell number | : | 750cells/well |
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| Medium | : | DMEM+4.5mg/mLGlc． |
| Additives | : | 15%Heat-inactive FCS 2mML-Glutamine 1%Non-essential amino acid 110μM2-Mercaptethanol |
| Culture days | : | 3 days |
PrimeSurfaceTM96U Stable/Uniform spheroid is generated. Efficiency of spheroid formation is excellent due to the prevention of cell adhesion on the plate surface. |
Almost of cells are adhesive or dead. |
Document reports for PrimeSurfaceTM
1. Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC. Aoki T, Ohnishi H, Oda Y, Tadokoro M, Sasao M, Kato H, Hattori K, Ohgushi H. Tissue Eng Part A. 2010 Jul;16(7):2197-206
2. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, Sasao M, Kubo Y, Hattori K, Saito S, Horimoto K, Yuba S, Ohgushi H., J Biol Chem. 2010 Jul 1.
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S-BIO Business Division
| Tel | : | +81-3-5462-4831 |
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| Fax | : | +81-3-5462-4835 |
